4 edition of Production & Characterization of Recombinant Rat Proopiomelanocortin 1-74 found in the catalog.
Production & Characterization of Recombinant Rat Proopiomelanocortin 1-74
by Leuven Univ Pr
Written in English
|Series||Acta Biomedica Lovaniensia, 216|
|The Physical Object|
|Number of Pages||109|
Regulation of the pro-opiomelanocortin mRNA levels in rat pituitary by dopaminergic compounds. Proc Natl Acad Sci U S A. Apr; 80 (8)– [PMC free article] Birnberg NC, Lissitzky JC, Hinman M, Herbert E. Glucocorticoids regulate proopiomelanocortin gene expression in vivo at the levels of transcription and secretion. Expression of proopiomelanocortin peptides in human dermal microvascular endothelial cells: evidence for a regulation by ultraviolet light and interleukin J. ; – Katahira M, Iwasaki Y, Aoki Y, Oiso Y, Saito H. Cytokine regulation of the rat proopiomelanocortin gene expression in .
Recombinant rat POMC was provided by C. Denef (University of Leuven, Leuven, Belgium). Synthesis of human POMC and POMC POMC and POMC were synthesized by standard solid phase chemistry on a TentaGel trityl-Leu-F-moc resin (Rapp Polymere; substitution level, mmol/g) at a scale of mmol on a A peptide. In addition to being a leading provider of high quality, affordable reagents for multicolor flow cytometry and ELISAs, BioLegend offers an extensive selection of purified human, mouse, and rat recombinant proteins, including cytokines, chemokines, and growth factors.
Rubinstein M, Mortrud M, Liu B, Low MJ. Rat and mouse proopiomelanocortin gene sequences target tissue-specific expression to the pituitary gland but not to the hypothalamus of transgenic mice. Neuroendocrinology ; – PubMed CrossRef Google Scholar. Weichang Zhou, Theodora Bibila, Konstantin Glazomitsky, Javier Montalyo, Christine Chan, Daniel DiStefano, Sonal Munshi, David Robinson, Barry Buckland and John Aunins, Large scale production of recombinant mouse and rat growth hormone by fed-batch GS-NSO cell cultures, Cytotechnology, /BF, 22, , (), ().
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Production & Characterization of Recombinant Rat Proopiomelanocortin (Acta Biomedica Lovaniensia, ): Medicine & Health Science Books @ BERT, C, V VANDE VIJVER, M ANDRIES, P VERHAERT, P PROOST, B DE VREESE, Jozef Van Beeumen, H VANKELECOM, and C DENEF.
“Production of Recombinant Rat Proopiomelanocortin and Characterization of Its Mitogenic Action on Pituitary Lactotrophs.” Molecular and Cellular Endocrinology (): –Cited by: Production of recombinant rat proopiomelanocortin and characterization of its mitogenic action on pituitary lactotrophs.
By C BERT, V VANDE VIJVER, M ANDRIES, P VERHAERT, P PROOST, B DE VREESE, Jozef Van Beeumen, H VANKELECOM and C DENEF. Cite. We report the production of biologically active recombinant rat Gly −2-Ser −1-POMC1–74 (rrPOMC1–74) in a prokaryotic expression polypeptide was produced as a fusion protein with glutathione-S-transferase (GST), using the pGEX-4T-1 vector and subsequently cleaved by acid sequencing, up to resi showed a correct primary structure including the Cited by: identified.
The recombinant rat 3 (IV)α NC1 antigen has nine amino acid spans that are consistent with antibody or T cell epitope that induce in EAG. s The research is carried out on the recombi-nant rat α3 (IV) NC1 production, purification, quantification, and characterization.
The circulation. Proopiomelanocortin has been shown to interact with melanocortin 4 receptor.   The endogenous agonists of melanocortin 4 receptor include α-MSH, β-MSH, γ-MSH, and ACTH. The fact that these are all cleavage products of POMC should suggest likely mechanisms of this interaction.
Day, in Encyclopedia of Neuroscience, The protein precursor known as proopiomelanocortin (POMC) holds within its structure numerous biologically active peptides, including adrenocorticotropic hormone (ACTH), β-lipotropin, β-endorphin, and peptides are proteolytically released from POMC by the proprotein convertases.
Differential cleavage of POMC occur, in the different. To date, pharmacological investigations mainly refer to recombinant hCRFBP. In contrast, the data on rat CRFBP (rCRFBP) are limited to the preliminary characterization of endogenous CRFBP from neuronal and astrocytic cells using oCRF, h/rCRF, α-helCRF 9–41 and h/rCRF 6–33 as ligands.
Since rat represents a major experimental animal. deﬁ ned chemical structures, therapeutic proteins pose a much more difﬁ cult production and characterization challenge. These proteins can be isolated from a variety of sources, including human, animal, or microorganism, but they are most often produced by biotechnological means, using recombinant DNA technologies.
Typically, the recombinant. High-level expression of milligrams of prohormones was achieved with the pET3c expression vector utilizing the T7 promoter for production of PE, pro-NPY, and POMC, as demonstrated by SDS–PAGE gel electrophoresis, Western blots, and 35 S-methionine labeling.
In addition, β-PT was expressed at high levels as fusion proteins with the maltose. Pintar JE, Schachter BS, Herman AB, Durgerian S, Krieger DT. Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis. Science. Aug 10; ()– Gizang-Ginsberg E, Wolgemuth DJ.
Tong, G. Pelletier, Role of dopamine in the regulation of proopiomelanocortin (POMC) mRNA levels in the arcuate nucleus and pituitary gland of the female rat as studied by in situ hybridization, Molecular Brain Research, /X(92), 15,(), ().
Production of recombinant rat proopiomelanocortin and characterization of its mitogenic action on pituitary lactotrophs. Article. Sep ; Christophe Bert. V Vande Vijver. Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts.
The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least nucleotides shorter than POMC messenger RNA found in the.
prohormone thiol protease (PTP) to process recombinant pro. neuropeptide Y, proopiomelanocortin, and proenkephalin. Pro-neuropeptide Y, proopiomelanocortin, and proenkepha- lin, as well as their peptide products, are co-localized with PTP in adrenal medulla and pituitary ([4,20,21]; Hook et.
It is now well established that the hormones corticotropin (ACTH) and β-lipotropin (β-LPH) are contained within a common precursor peptide called proopiomelanocortin (POMC), which is synthesized.
Abstract. Proopiomelanocortin (POMC) is the name given to the protein precursor for both ß-endorphin and adrenocorticotropin (ACTH).
Although the proof of a common precursor for these important peptides was provided by chemical evidence (1–3), the elucidation of the exact structure of POMC and the prediction of other biologically important peptides in the same precursor was one of the first. Bradford assay: Bradford assay is a colorimetric assay based on the interaction between Coomassie brilliant blue and arginine and aromatic residues in your protein, resulting in a shift of the absorption maximum of the dye from to general, the absorbance of a series of known concentrations of a standard protein, generally BSA, is measured to create a standard curve, which can be.
Characterization of Proopiomelanocortin mRNA Detected by in situ Hybridization Jeffrey E. Kelsey, Stanley J. Watson, Sharon Burke, Huda Akil, and James L. Roberts*. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants.
Pintar JE, Schachter BS, Herman AB, Durgerian S, Krieger DT. Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis. Science. Aug 10; ()– Jingami H, Nakanishi S, Imura H, Numa S. Tissue distribution of messenger RNAs coding for opioid peptide precursors and related RNA.
Eur J Biochem.Production of recombinant rat proopiomelanocortin and characterization of its mitogenic action on pituitary lactotrophs. Article. Sep .recombinant protein or 50mg purified antibody guaranteed 8 weeks Staring from $8, YeastHighTM Customizable production up to L 8 weeks Quote From sequence to purified protein - gene synthesis included!